Innate and adaptive immune responses subsequent to ischemia-reperfusion injury in the kidney

25 juin 2014

Auteurs : C. Denecke, S.G. Tullius
Référence : Prog Urol, 2014, 24, S13, suppl. S1




 




Introduction


In the past years, transplantation research has emphasized on understanding the mechanisms of innate immune activation and their initiation of alloimmune responses in organ transplantation. While mechanisms of acute rejections (AR) have been well elucidated and treatment has been effective, innate immune activation early after transplantation has not been targeted successfully, yet. Thus, understanding the consequences of IRI on innate and adaptive immune activation appears critical for an early interference with with the activated immune cascade.


The innate immunity acts as the first line of defense against foreign tissue by detecting so called damage-associated or pathogen - associated molecular patterns (DAMP's, PAMP's) which bind to pattern recognition receptors (PRR) on APC's and other innate immune cells. Mechanical, thermal and pathogen induced cell damage cause a nonspecific inflammatory response including TLR activation and proinflammatory cytokine release, thus starting a cascade of neutrophil, DC and T-cell activation [1]. Indeed, surgery itself, brain death and ischemia have all been shown to activate innate immune responses [1, 2, 3]. Thus, IRI serves as a non-specific inflammatory injury which elicits a coordinated alloimmune specific response against the graft.


Ischemia Reperfusion injury


IRI is characterized by ATP depletion, lack of glycogen and oxygen supply, all resulting into metabolic changes. As a consequence, renal tubular epitihelial cells are injured, tissue resident leukocytes are activated and endothelial cell function is impaired leading to vascular leakage and interstitial edema. Following the upregulation of adhesion molecules (ICAM-1, P-Selectin and others), endo- and epithelial damage is furthermore accelerated by complement activation with subsequent increased cytokine levels resulting into the adhesion of leukocytes to the endothelium capturing red blood cells and platelets in turn [4, 5, 6]. Tubular epithelial cells, on the other hand, upregulate TLR-2 and TLR-4 and internalize the complement inhibitory factor Crry which, in turn, causes deposition of complement, production of chemokines and recrution of polymorph nuclear leukocytes (PMN's) [7,8].


Besides, reactive oxygen species (ROS) such as superoxide, peroxynitrite or H2 O2 derived hydroxyl radicals facilitate further DNA damage and activate an ADP polymerase (PARP- 1) causing considerable tissue injury. Various inflammatory cells express NADPH oxidases and act as a source of ROS, neutrophils being the most abundant [9]. Arandomized clinical trial in kidney transplant patients demonstrated a beneficial effect of the free radical scavenger superoxide dismutase [10]. Amelioration of endothelial cell damage caused by free oxygen radicals lead to significantly improved long-term graft survival and reduced rates of acute rejection (AR) suggesting a causal relation between innate immune injury and chronic immune stimulation.


Furthermore, cell death programs such as apoptosis, autophagy-associated cell death and necrosis are initiated subsequent to IRI [11]. While necrosis causes further immune stimulation, apoptosis is supposed to cause less inflammation. Yet, there are recent reports of an apoptosis associated stimulation of monocytes and macrophages presumably also leading to innate immune activation [12].


Activation of innate immune cells


The innate immune response as a first line response is carried out by neutrophils, macrophages, Dendritic Cells (DC), NK and NKT-cells and T-cells. Following reperfusion, neutrophils adhere to the endothelium and migrate into the tissue. Neutrophils react immediately to unspecific injury, infiltrate the tissue and release proteases, oxygen-free radicals through degranulation and start producing proinflammatory cytokines such as IL-4, IL-6, IFNγ, TNFα [13]. In line with those findings, neutrophil depletion protected mice from IRI [14].


Similarly, macrophages exhibiting an activated phenotype and producing proinflammatory cytokines (IL-1a , IL-6 IL-12, TNFα) can be found at very early stages of IRI [15,16]. Migration of monocytes/macrophages is mediated by various chemokines/chemokine receptors, i.e. CX3CR1 or CCR2. Of note, CCR2 - deficient macrophages were unable to mediate injury [15,17]. Likewise, blockade of CX3CR1 and depletion of macrophages abrogated renal IRI showing that monocyts/macrophages play a key role in initiating an early innate response after acute kidney injury [18,19].


Besides, platelets interacting with endothelial cells become activated and cause subsequent activation of plasma factor XII, thereby enhancing the coagulative and inflammatory milieu [20]. The pro-inflammatory cytokine milieu further results into the activation of both, direct and indirect pathways of the complement system. Complement components are released both, systemically (liver, endothelium) as well as locally in the kidney and the deposition of C3, C6 and Mannose-binding Lectin can be detected during reperfusion and after transplantation [21,22]. Complement activation is not only a part innate immune responses but also impacts adaptive immunity. While B-cells are stimulated to produce antibodies, T-cell differentiation is influenced by complement components such as C3a, C5a or decay-accelerating factors modulating T-cell immunity [23, 24, 25].


NK cells are an essential part of the innate immune surveillance. NK cells have the capacity to extinct pathogens through granzyme, perforin and FasL dependent cell cytotoxicity. NK cells have been shown to play a central role in renal IRI as they can be detected within 4hrs after reperfusion in the kidney. Perforin dependent killing of tubular cells by NK cells is a major pathway of renal IRI [26]. Moreover, expression of CD137 on NK cells and its ligand on tubular epithelial cells seems crucial for the chemokine mediated recruitment of neutrophils to the site of inflammation [27]. Adoptive transfer of wild-type NK-cells into CD137 deficient mice, in turn, restored IRI, thus demonstrating a crucial role of NK cells in renal IRI. Besides, upon activation and IFNγ secretion in the draining lymph node, NK cells are able to provide fast priming of a Th1 response [28]. Of note, NK-cells interact with the adaptive immune response in many ways, especially through a reciprocal crosstalk with DC's [29]. Moreover, NK cells induce the maturation of DC, which in turn are potent inducers of T-cell activation. On the other hand, NK cells may induce lysis of an excessive amount of immature DC's, thereby preventing an unnecessary T-cell response [30]. Although NK cells are important in the early reperfusion phase and subsequent activation of both, innate and adaptive immune activation, they are unable to reject graft on their own as demonstrated in a murine skin transplant model [31].


DCs can be found within less than an hour after reperfusion of the graft and migrate thereafter into local lymph nodes presenting antigens to adaptive immune cells [32,33]. Following IRI, DCs undergo an antigen-independent maturation process induced by DAMPs and PAMPs. Of note, elevated numbers of DCs can be observed after syngenic renal transplantation [34]. Moreover, renal DCs aggravate IRI through the production of pro-inflammatory mediators such as TNFα, IL-6, MCP-1 and RANTES while DC depletion ameliorated local inflammation [35]. At the same time, DCs are a key link between innate and adaptive immune activation. Upon maturation, they are capable of clonal expansion of an antigen specific immune response. DC maturation is induced following TLR engagement leading to the expression of a pro-inflammatory receptor profile (CCR4, CXCR4, CCR7), the upregulation of costimulatory molecules (CD80, CD86) and MHC Class II expression. In renal transplantation, oxidative stress induced by brain death caused activation of donor DCs with a subsequent activation of recipient T-cells [36].


Toll-like receptors


Innate immune cells such as monocytes, macrophages, and DCs become activated following PRR engagement, i.e. scavengers and Toll - like receptors (TLR). There is strong evidence that TLRs are essential in initiating the ischemia reperfusion injury.


The TLR family consists of 12 receptors in mice and 10 in humans which are expressed on innate immune cells; TLR and are activated through endogenous ligands (DAMPs) released from necrotic tissue (ATP, heat-shock proteins, high mobility group box - 1 (HMGB-1) or extracellular matrix (fibrinogen, hyaluronic acid, heparin sulfate) [37,38]. Following activation, downstream TLR signaling (except TLR3) is mediated via the MyD88 (myeloid differentiation primary response gene 88) and IFN regulatory factor 3 dependent pathway resulting in NFkB activation and gene induction of various chemokines and cytokines [39]. These changes have been shown to be critical for the activation of naïve T-cells, thereby driving pathogen specific T-cell responses [40]. In mouse models of liver IRI, TLR-4 rather than TLR-2 has been identified as a MyD88 independent key mechanism mediating hepatic inflammation [38,41,42]. In contrast, TLR-2 and TLR-4 have been shown to play a role in murine cardiac IRI, with mechanistic implications of HMBG-1, MyD88 and TRIF in TLR-4 activation and signaling [43,44].


Likewise, TLR 4 signaling plays a major role in IRI after experimental kidney transplantation since both MyD88 and TLR4 deficient mice did not develop IRI [45]. Similarly, renal TLR 2 was shown to initiate inflammatory responses after IRI while TLR-2 antisense treatment protected mice from renal dysfunction and inflammatory effects [46].


In clinical transplantation, TLR 4 expression has been associated with early graft failure in patients undergoing kidney transplantation. Besides the fact that HMGB-1 expression was significantly higher in kidneys from deceased donors compared to those from living donors, kidneys with a functional TLR4 loss had a higher immediate graft function rate than kidneys with a TLR4 wild-type allele [47].


Interestingly, it has been demonstrated that MyD88 deficiency promotes allograft tolerance in a murine kidney transplant model, thereby suggesting a role of an altered Th17/Treg ratio in MyD88 deficient recipients [48]. Similarly, in a murine skin graft model, MyD88 associated abrogation of transplantation tolerance was observed [49]. Moreover, TLR activation has been shown to block the suppressive effects of regulatory T-cells, thus accelerating alloimmune responses [50]. These data highlight the importance of TLR activation and MyD88 signalling in IRI injury and its subsequent impact on the adaptive immune response.


T-cell activation following IRI


While activation of the innate immune system takes places within minutes, the adaptive immune response is generated after a few days. T-cells involved in either antigen-specific or antigen-unspecific responses play a key role in kidney IRI [51]. In contrast, mice lacking T- or B-cells are protected from IRI [52,53]. T-cells can be activated through free oxygen radicals, cytokines or RANTES in an Ag-unspecific fashion while Ag dependent T-cell activation involves presentation of antigensby B-cells, DCs or endothelial cells (reviewed in 54). Consequently, costimulatory blockade inhibiting T-cell activation ameliorated IRI in animal models [55, 56, 57]. It is well established that elevated levels of pro-inflammatory cytokines such as IFNγ, TNFα, IL-1β, IL-2 or IL-6 are involved in T-cell mediated IRI [58]. IFNγ seems to be playing a critical role as splenic T-cells contained more IFNγ after IRI while IFNγ deficient mice were protected from IRI [59, 60, 61]. The role of T-cells in IRI is furthermore supported by findings which demonstrate that various immunosuppressant attenuate IRI via T-cell inhibition. For instance, FTY720, FK506 and Mycophenolate Mofetil (MMF) decreased IRI in experimental transplant models [62, 63, 64].


Moreover, activation of T-cells also translates into longterm immunological and morphological changes subsequent to renal IRI. 6 weeks after murine renal ischemia, morphological deterioration had been associated with the accumulation of neutrophil and CD4+ T-cell infiltrates. Interestingly, higher numbers of splenic IFNγ positive T-cells suggested a systemic T-cell activation late after IRI [60].


Yet, T-cells may also have protective effects in renal IRI. Regulatory T-cells were shown to exert beneficial effects via a IL-10 dependent antagonization of TNFα and IFNγ secreted by inflammatory cells in an experimental stroke model [65]. Mice deficient of IL-4 known to regulate Th2 differentiation but not IL-12 had a significantly worse graft function and advanced histological alterations suggesting deleterious effects of Th1 cells and beneficial effects of Th2 cells in renal IRI [66]. The adverse role of Th1 cells in renal IRI is furthermore supported by the observation of a predominant Th1 response in kidney transplant patients with Delayed Graft function [67]. However, data on the role of Th2 cells remain controversial since other studies have reported on opposite effects of Th2 associated cytokines in IRI [68]. Interestingly, CD8 T-cells have also been shown to augment the immediate innate response prior to T-cell priming [69]. Hours after graft reperfusion, IFNγ producing CD8 T-cells were shown to regulate early inflammation through the activation of PMNs and endothelial cells.


Recently, IL-17 producing γδ Tcells have been shown to be detrimental in brain ischemia [70]. In this model, depletion of γδ Tcells attenuated IRI, suggesting that T-cell depletion may also be beneficial in transplantation associated IRI.


These studies highlight the multi-faceted role of T-cell subsets during all aspects of immune responses subsequent to IRI.


Targeting IRI


Ischemic preconditioning implies a first period of organ ischemia "tolerizing" the graft to a subsequent second ischemia period. Ischemic preconditioning in patients undergoing major liver resection was superior over portal clamping and continuous inflow occlusion in protecting from postoperative liver injury [71]. In liver transplantation, ischemic preconditioning of the donor graft has been linked to lower ASAT and ALAT levels but has not improved early organ function [72].


Ischemic preconditioning has been successful in animal models of kidney transplantation, however it has not been translated into clinical transplantation yet. In a recent systematic review and meta-analysis of kidney animal models, ischemic preconditioning has been effective in reducing IRI, expecially if conducted >24h prior to ischemia [73]. Yet, the transfer into the clinical setting may be difficult due to the heterogeneity of data, the influence of gender and animal strain differences as well as the unknown impact of co-morbidities and medications on the effects of ischemic preconditioning [74].


Nitric oxide (NO) and its effects on vascular tone and endothelial function have been utilized as therapeutic approaches. Patients inhaling NO during liver transplantation had a better restauration of liver function associated with a decreased apoptosis of hepatocytes [74]. Similiarly, administration of nitrite stimulating NO signaling attenuated IRI in a rat kidney transplant model [75].


Adenosine is a well-known anti-inflammatory molecule, a modulator of lymphocyte responses and has been implicated in improved outcomes after acute kidney injury [76]. Activation of the Adenosine receptor A2a R expressed on DCs lead to inhibition of NF&kgr;B and the transcripition of pro-inflammatory cytokines. Recently, it has been shown that administration of the selective A2a R agonist ATL313 attenuated renal IRI via tolerizing effects on DCs [77]. Similarly, ATL313 treatment at the time of reperfusion protected mice from liver IRI via effects on bone marrow derived cells [78]. Furthermore, ATL313 therapy also reduced alloimmune response after transplantation as skin allograft survival had been enhanced in mice [79].


Toll-like receptors are essential in mediating leukocyte activation and play a critical role in instigating early inflammatory response. Selective TLR 4 blockade by TAK-242 has been effective in attenuating early acute kidney injury in experimental models [80]. TLR 2 and 4 suppression was further implicated in mediating the protective effects of Serp-1, a serine protease inhibitor with anti-inflammatory capacities in a cardiac transplant model [81]. Interestingly, the combination of Serp-1 and subtherapeutic Cyclosporin doses lead to indefinite graft survival in a fully mismatched transplant model, indicating a role of Serp-1 in translating innate into adaptive immune responses. In contrast, a phase II clinical study in septic ICU patients treated with TAK 242 showed only a non-significant reduction in 28 day mortality rate [82].


Erythropoetin (EPO) has also been tested in preventing renal IRI. A study by Imamura et al. demonstrated that EPO increased hypoxia inducible factor-1alpha (HIF-1α) expression and attenuated tubular hypoxia [83]. In an additional study, it has been shown that carbamylated EPO promoted tubular cell proliferation and decreased tubular apoptosis in a rat model of renal IRI [84]. The protective effects of heme oxygenase 1 (HO-1) in renal IRI have also been tested. HO-1 induction by Cobalt-Proto Porphyrin was associated with significantly prolonged graft survival in a rat renal transplant model [85]. In a clinically relevant transplant model, HO-1 induction further attenuated the consequences of donor brain death and increased graft survival [86].


Targeting apoptosis has been another attempt to attenuate IRI. In rodent kidneys, IRI was significantly reduced in mice lacking TSP-1, a matricellular protein causing apoptosis [87].


Conclusion


Dissecting mechanisms of IRI has prompted studies to improve cellular resistance to hypoxia. The growing interest in innate immune responses as the first line of attack following organ transplantation has broadened the understanding of a continuous immunological cascade from IRI to chronic adaptive immune responses. While T-cell research has been in the center of interest for many years, elucidating the functional roles of monocytes/macrophages, NK-cells, DCs and their activation patterns has shed light on novel treatment options. Experimental studies using knock-out animals have revealed interesting opportunities to selectively interfere with the early innate response, thus attenuating antigen-specific immune activation. A better understanding of the role of specific T-cell subsets in IRI, may harbor further potential for cell therapies. While it has become clear that innate responses subsequqent to IRI are playing a critical role for the success in organ transplantation, clinical studies in addition to an improved understanding of mechanisms are warranted.


Disclosure of interest


The authors have no conflicts of interest to declare in relation to this review.



References



Paterson H.M., Murphy T.J., Purcell E.J., Shelley O., Kriynovich S.J., Lien E., et al. Injury primes the innate immune system for enhanced toll-like receptor reactivity J Immunol 2003 ;  171 : 1473
Tullius S.G., Reutzel-Selke A., Egermann F., Nieminen-Kelhä M., Jonas S., Bechstein W.O., et al. Contribution of prolonged ischemia and donor age to chronic renal allograft dysfunction J Am Soc Nephrol 2000 ;  11 : 1317
Matzinger P. The danger model: A renewed sense of self Science 2002 ;  296 : 301 [cross-ref]
Rosenberger P., Schwab J.M., Mirakaj V., Masekowsky E., Mager A., Morote-Garcia J.C., et al. Hypoxia-inducible factor-dependent induction of netrin-1 dampens inflammation caused by hypoxia Nat. Immunol 2009 ;  10 : 195-202 [cross-ref]
Eltzschig H.K., Collard C.D. Vascular ischaemia and reperfusion injury Br Med Bull 2004 ;  70 : 71-86 [cross-ref]
Hidalgo A., Chang J., Jang J.E., Peired A.J., Chiang E.Y., Frenette P.S. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury Nat Med 2009 ;  15 : 384-391 [cross-ref]
Thurman J.M., Ljubanovic D., Royer P.A., Kraus D.M., Molina H., Barry N.P., et al. Altered renal tubular expression of the complement inhibitor Crry permits complement activation after ischemia/reperfusion J Clin Invest 2006 ;  116 : 357-368
Thurman J.M., Lenderink A.M., Royer P.A., Coleman K.E., Zhou J., Lambris J.D., et al. C3a is required for the production of CXC chemokines by tubular epithelial cells after renal ischemia/reperfusion J Immunol 2007 ;  178 : 1819-1828 [cross-ref]
Kahles T., Brandes R.P. NADPH oxidases as therapeutic targets in ischemic stroke Cell Mol Life Sci 2012 ;  69 (14) : 2345-2363Jul; Epub 2012 May 23 Review.
 [cross-ref]
Land W., Schneeberger H., Schleibner S., Illner W.D., Abendroth D., Rutili G., et al. The beneficial effect of human recombinant superoxide dismutase on acute and chronic rejection events in recipients of cadaveric renal transplants Transplantation 1994 ;  57 : 211 [cross-ref]
Hotchkiss R.S., Strasser A., McDunn J.E., Swanson P.E., et al. Cell death N. Engl. J. Med 2009 ;  361 : 1570-1583 [cross-ref]
Chekeni F.B., Elliott M.R., Sandilos J.K., Walk S.F., Kinchen J.M., Lazarowski E.R., et al. Pannexin 1 channels mediate ‘find-me'signal release and membrane permeability during apoptosis Nature 2010 ;  467 : 863-867 [cross-ref]
Awad A.S., Rouse M., Huang L., Vergis A.L., Reutershan J., Cathro H.P., et al. Okusa Compartmentalization of neutrophils in the kidney and lung following acute ischemic kidney injury Kidney Int 2009 ;  75 (7) : 689-698April.
 [cross-ref]
Kelly K.J., Williams W.W., Colvin R.B., Meehan S.M., Springer T.A., Gutierrez-Ramos J.C., et al. Intercellular adhesion molecule-1- deficient mice are protected against ischemic renal injury J Clin Invest 1996 ;  97 : 1056-1063 [cross-ref]
Li L., Huang L., Sung S.S., Vergis A.L., Rosin D.L., Rose C.E., et al. The chemokine receptors CCR2 and CX3CR1 mediate monocyte/macrophage trafficking in kidney ischemia-reperfusion injury Kidney Int 2008 ;  74 : 1526-1537 [cross-ref]
Day Y.J., Huang L., Ye H., Li L., Linden J., Okusa M.D., et al. Renal ischemia-reperfusion injury and adenosine 2A receptor-mediated tissue protection: role of macrophages Am J Physiol Renal Physiol 2005 ;  288 : F722-F731
Furuichi K., Wada T., Iwata Y., Kitagawa K., Kobayashi K., Hashimoto H., et al. CCR2 Signaling Contributes to Ischemia-Reperfusion Injury in Kidney J Am Soc Nephrol 2003 ;  14 (10) : 2503-2515 [cross-ref]
Jo S.K., Sung S.A., Cho W.Y., Go K.J., Kim H.K. Macrophages contribute to the initiation of ischaemic acute renal failure in rats Nephrol Dial Transplant 2006 ;  21 (5) : 1231-1239 [cross-ref]
Oh D.J., Dursun B., He Z., Lu L., Hoke T.S., Ljubanovic D., et al. Fractalkine receptor (CX3CR1) inhibition is protective against ischemic acute renal failure in mice Am J Physiol Renal Physiol 2008 ;  294 (1) : F264-F271
Moser M., Nieswandt B., Ussar S., Pozgajova M., Fässler R. Kindlin-3 is essential for integrin activation and platelet aggregation Nat Med 2008 ;  14 : 325-330 [cross-ref]
de Vries B., Walter S.J., Peutz-Kootstra C.J., Wolfs T.G., van Heurn L.W., Buurman W.A., et al. The mannose-binding lectin-pathway is involved in complement activation in the course of renal ischemiareperfusion injury Am J Pathol 2004 ;  165 : 1677-1688 [cross-ref]
Pratt J.R., Basheer S.A., Sacks S.H. Local synthesis of complement component C3 regulates acute renal transplant rejection Nat Med 2002 ;  8 : 582-587 [cross-ref]
Fearon D.T., Carroll M.C. Regulation of B lymphocyte responses to foreign and self-antigens by the CD19/CD21 complex Annu Rev Immunol 2000 ;  18 : 393-422 [cross-ref]
Hawlisch H., Wills-Karp M., Karp C.L., Köhl J. The anaphylatoxins bridge innate and adaptive immune responses in allergic asthma Mol Immunol 2004 ;  41 : 123-131 [cross-ref]
Heeger P.S., Lalli P.N., Lin F., Valujskikh A., Liu J., Muqim N., et al. Decay-accelerating factor modulates induction of T cell immunity J Exp Med 2005 ;  201 : 1523-1530 [cross-ref]
Zhang Z.X., Wang S., Huang X., Min W.P., Sun H., Liu W., Garcia B., et al. NK cells induce apoptosis in tubular epithelial cells and contribute to renal ischemia-reperfusion injury J Immunol 2008 ;  181 : 7489-7498 [cross-ref]
Kim H.J., Lee J.S., Kim J.D., Cha H.J., Kim A., Lee S.K., et al. Reverse signaling through the costimulatory ligand CD137L in epithelial cells is essential for natural killer cell-mediated acute tissue inflammation Proc Natl Acad Sci USA. 2012 ;  109 (1) : E13-E22Jan 3.
Martín-Fontecha A., Thomsen L.L., Brett S., Gerard C., Lipp M., Lanzavecchia A., et al. Induced recruitment of NK cells to lymph nodes provides IFN-gamma for TH1 priming Nat Immunol 2004 ;  5 : 1260
Raulet D.H. Interplay of natural killer cells and their receptors with the adaptive immune response Nat Immunol 2004 ;  5 : 996 [cross-ref]
Piccioli D., Sbrana S., Melandri E., Vallante N.M. Contactdependent stimulation and inhibition of dendritic cells by natural killer cells J. Exp. Med 2002 ;  195 : 335-341 [cross-ref]
Zijlstra M., Auchincloss H., Loring J.M., Chase C.M., Russel P.S., Jaenisch R. Skin graft rejection by beta 2-microglobulin-defi- cient mice J Exp Med 1992 ;  175 : 885-893 [cross-ref]
Zhou T., Sun G.Z., Zhang M.J., Chen J.L., Zhang D.Q., Hu Q.S., et al. Role of adhesion molecules and dendritic cells in rat hepatic/renal ischemia-reperfusion injury and anti-adhesive intervention with anti-P-selectin lectin-EGF domain monoclonal antibody World J Gastroenterol 2005 ;  11 : 1005-1010
Dong X., Swaminathan S., Bachman L.A., Croatt A.J., Nath K.A., Griffin M.D. Antigen presentation by dendritic cells in renal lymph nodes is linked to systemic and local injury to the kidney Kidney Int 2005 ;  68 : 1096-1108 [cross-ref]
Penfield J.G., Dawidson I.A., Ar'Rajab A., Kielar M.A., Jeyarajah D.R., Lu C.Y. Syngeneic renal transplantation increases the number of renal dendritic cells in the rat Transpl Immunol 1999 ;  7 : 197-200 [cross-ref]
Dong X., Swaminathan S., Bachman L.A., Croatt A.J., Nath K.A., Griffin M.D. Resident dendritic cells are the predominant TNFsecreting cell in early renal ischemia-reperfusion injury Kidney Int 2007 ;  71 : 619-628 [cross-ref]
Land W.G. The role of postischemic reperfusion injury and other nonantigen-dependent inflammatory pathways in transplantation Transplantation 2005 ;  79 : 505 [cross-ref]
Kawai T., Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors Nat Immunol 2010 ;  11 (5) : 373-384May.
 [cross-ref]
Tsung A., Hoffman R.A., Izuishi K., Critcholow N.D., Nakao A., Chan M.H., et al. Hepatic ischemia/reperfusion injury involves functional TLR4 signaling in nonparenchymal cells J Immunol 2005 ;  175 : 7661-7668 [cross-ref]
Chen G.Y., Nunez G. Sterile inflammation: sensing and reacting to damage Nat. Rev. Immunol. 2010 ;  10 : 826-837 [cross-ref]
El Shikh M.E., El Sayed R.M., Wu Y., Szakal A.K., Tew J.G. TLR4 on follicular dendritic cells: An activation pathway that promotes accessory activity J Immunol 2007 ;  179 : 4444
Shen X.-D., Ke B., Zhai Y., Gao F., Busuttil R.W., Cheng G., et al. Toll-like receptor and heme oxygenase-1 signaling in hepatic ischemia/reperfusion injury Am J Trans 2005 ;  5 : 1793 [cross-ref]
Zhai Y., Shen X.D., O'Connell R., Gao F., Lassman C., Busuttil R.W., et al. Cutting edge: TLR4 activation mediates liver ischemia/reperfusion inflammatory response via IFN regulatory factor 3-dependent MyD88-independent pathway J Immunol 2004 ;  173 : 7115-7119 [cross-ref]
Shishido T., Nozaki N., Yamaguchi S., Shibata Y., Nitobe J., Miyamoto T., et al. Toll-like receptor-2 modulates ventricular remodeling after myocardial infarction Circulation 2003 ;  108 : 2905 [cross-ref]
Kaczorowski D.J., Nakao A., Vallabhaneni R., Mollen K.P., Sugimoto R., Kohmoto J., et al. Mechanisms of toll-like receptor 4 (TLR4)- mediated inflammation after cold ischemia/reperfusion in the heart Transplantation 2009 ;  87 : 1455 [cross-ref]
Wu H., Cheng G., Wyburn K.R., Yin J., Bertolino P., Eris J.M., et al. TLR4 activation mediates kidney ischemia/reperfusion injury J. Clin. Invest. 2007 ;  117 : 2847-2859 [cross-ref]
Leemans J.C., Stokman G., Claessen N., Rouschop K.M., Teske G.J., Kirschning C.J., et al. Renal-associated TLR2 mediates ischemia/reperfusion injury in the kidney J. Clin. Invest 2005 ;  115 : 2894-2903 [cross-ref]
Kruger B., Krick S., Dhillon N., Lerner S.M., Ames S., Bromberg J.S., et al. Donor Toll-like receptor 4 contributes to ischemia and reperfusion injury following human kidney transplantation Proc. Natl. Acad. Sci. USA 2009 ;  106 : 3390-3395 [cross-ref]
Wu H., Noordmans G.A., O'Brien M.R., Ma J., Zhao C.Y., Zhang G.Y., et al. Absence of MyD88 signaling induces donor-specific kidney allograft tolerance J Am Soc Nephrol 2012 ;  23 (10) : 1701-1716Oct; Epub 2012 Aug 9.
 [cross-ref]
Chen L., Wang T., Zhou P., Ma L., Yin D., Shen J., et al. TLR engagement prevents transplantation tolerance Am J Transplant 2006 ;  6 : 2282 [cross-ref]
Pasare C., Medzhitov R. Toll pathway-dependent blockade of CD4_CD25_ T cell-mediated suppression by dendritic cells Science 2003 ;  299 : 1033 [cross-ref]
Satpute S.R., Park J.M., Jang H.R., Agreda P., Liu M., Gandolfo M.T., et al. The role for T cell repertoire/antigen-specific interactions in experimental kidney ischemia reperfusion injury J. Immunol 2009 ;  183 : 984-992 [cross-ref]
Burne-Taney M.J., Ascon D.B., Daniels F., Racusen L., Baldwin W., Rabb H. J. Immunol 2003 ;  171 : 3210-3215B cell deficiency confers protection from renal ischemia reperfusion injury.
 [cross-ref]
Yokota N., Daniels F., Crosson J., Rabb H., et al. Protective effect of T cell depletion in murine renal ischemia-reperfusion injury Transplantation 2002 ;  74 : 759-763 [cross-ref]
Huang Y., Rabb H., Womer K.L. Ischemia-reperfusion and immediate T cell responses Cell. Immunol 2007 ;  248 : 4-11 [cross-ref]
Takada M., Chandraker A., Nadeau K.C., Sayegh M.H., Tilney N.L. The role of the B7 costimulatory pathway in experimental cold ischemia/reperfusion injury J Clin Invest 1997 ;  100 : 1199-1203 [cross-ref]
De Greef K.E., Ysebaert D.K., Dauwe S., Persy V., Vercauteren S.R., Mey D., et al. Anti-B7-1 blocks mononuclear cell adherence in vasa recta after ischemia Kidney Int 2001 ;  60 : 1415-1427 [cross-ref]
Ishikawa M., Vowinkel T., Stokes K.Y., Arumugam T.V., Yilmaz G., Nanda A., et al. CD40/CD40 ligand signaling in mouse cerebral microvasculature after focal ischemia/reperfusion Circulation 2005 ;  111 : 1690-1696 [cross-ref]
Boros P., Bromberg J.S. New cellular and molecular immune pathways in ischemia/reperfusion injury Am J Transplant 2006 ;  6 : 652-658 [cross-ref]
Burne-Taney M.J., Yokota N., Rabb H. Persistent renal and extrarenal immune changes after severe ischemic injury Kidney Int 2005 ;  67 : 1002-1009 [cross-ref]
Day Y.J., Huang L., Ye H., Li L., Linden J., Okusa M.D., et al. Renal ischemia-reperfusion injury and adenosine 2A receptor-mediated tissue protection: the role of CD4+ T cells and IFN-gamma J Immunol 2006 ;  176 : 3108-3114 [cross-ref]
Burne M.J., Daniels F., El Ghandour A., Mauiyyedi S., Colvin R.B., O'Donnell M.P., et al. Identification of the CD4+ T cell as a major pathogenic factor in ischemic acute renal failure J Clin Invest 2001 ;  108 : 1283-1290 [cross-ref]
Garcia-Criado F.J., Lozano-Sanchez F., Fernandez-Regalado J., Valdunciel-Garcia J.J., Parreno-Manchado F., Silva-Benito I., et al. Possible tacrolimus action mechanisms in its protector effects on ischemia-reperfusion injury Transplantation 1998 ;  66 : 942-943 [cross-ref]
Ysebaert D.K., De Greef K.E., Vercauteren S.R., Verhulst A., Kockx M., Verpooten G.A., et al. Effect of immunosuppression on damage, leukocyte infiltration, and regeneration after severe warm ischemia/reperfusion renal injury Kidney Int 2003 ;  64 : 864-873 [cross-ref]
Dragun D., Bohler T., Nieminen-Kelha M., Waiser J., Schneider W., Haller H., et al. FTY720-induced lymphocyte homing modulates post-transplant preservation/reperfusion injury Kidney Int 2004 ;  65 : 1076-1083 [cross-ref]
Liesz A., Suri-Payer E., Veltkamp C., Doerr H., Sommer C., Rivest S., et al. Regulatory T cells are key cerebroprotective immunomodulators in acute experimental stroke Nat. Med 2009 ;  15 : 192-199
Marques V.P., Gonçalves G.M., Feitoza C.Q., Cenedeze M.A., Fernandes Bertocchi A.P., Damiao M.J., et al. Influence of TH1/TH2 switched immune response on renal ischemia-reperfusion injury Nephron Exp Nephrol 2006 ;  104 (1) : e48-e56.
 [inter-ref]
Loverre A., Divella C., Castellano G., Tataranni T., Zaza G., Rossini M., et al. T helper 1, 2 and 17 cell subsets in renal transplant patients with delayed graft function Transpl Int 2011 ;  24 (3) : 233-242Mar.
 [cross-ref]
Nussler N.C., Muller A.R., Weidenbach H., Vergopoulos A., Platz K.P., Volk H.D., et al. IL-10 increases tissue injury after selective intestinal ischemia/reperfusion Ann Surg 2003 ;  238 : 49-58 [cross-ref]
El-Sawy T., Miura M., Fairchild R. Early T cell response to allografts occurring prior to alloantigen priming up-regulates innatemediated nflammation and graft necrosis Am J Pathol 2004 ;  165 : 147-157 [cross-ref]
Shichita T., Sugiyama Y., Ooboshi H., Sugimori H., Nakagawa R., Takada I., et al. Pivotal role of cerebral interleukin-17-producing gd T cells in the delayed phase of ischemic brain injury Nat. Med 2009 ;  15 : 946-950 [cross-ref]
Petrowsky H., McCormack L., Trujillo M., Selzner M., Jochum W., Clavien P.A., et al. A prospective, randomized, controlled trial comparing intermittent portal triad clamping versus ischemic preconditioning with continuous clamping for major liver resection Ann. Surg 2006 ;  244 : 921-928discussion 928-30.
 [cross-ref]
Azoulay D., Del Gaudio M., Andreani P., Ichai P., Sebag M., Adam R., et al. Effects of 10 minutes of ischemic preconditioning of the cadaveric liver on the graft's preservation and function: the ying and the yang Ann. Surg 2005 ;  242 : 133-139 [cross-ref]
Wever K.E., Menting T.P., Rovers M., van der Vliet J.A., Rongen G.A., Masereeuw R., et al. Ischemic Preconditioning in the Animal Kidney, a Systematic Review and Meta-Analysis PLoS One 2012 ;  7 (2) : e63229.
Lang J.D., Teng X., Chumley P., Crawford J.H., Isbell T.S., Chacko B.K., et al. Inhaled NO accelerates restoration of liver function in adults following orthotopic liver transplantation J. Clin. Invest 2007 ;  117 : 2583-2591 [cross-ref]
Kelpke S.S., Chen B., Bradley K.M., Teng X., Chumley P., Brandon A., et al. Sodium nitrite protects against kidney injury induced by brain death and improves post-transplant function Kidney Int 2012 ;  82 (3) : 304-313Aug; doi: 10.1038/ki.2012.116. Epub 2012 Apr 25.
 [cross-ref]
Huang S., Apasov S., Koshiba M., Sitkovsky M., et al. Role of A2a extracellular adenosine receptormediated signaling in adenosine- mediated inhibition of T-cell activation and expansion Blood 1997 ;  90 (4) : 1600-1610
Li L., Huang L., Ye H., Song S.P., Bajwa A., Lee S.J., et al. Dendritic cells tolerized with adenosine A2AR agonist attenuate acute kidney injury J Clin Invest 2012 ;  122 (11) : 3931-3942 [cross-ref]
Day Y.J., Li Y., Rieger J.M., Ramos S.I., Okusa M.D., Linden J., et al. A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury J Immunol 2005 ;  174 (8) : 5040-5046 [cross-ref]
Sevigny C.P., Li L., Awad A.S., Huang L., McDuffie M., Linden J., et al. Activation of adenosine 2A receptors attenuates allograft rejection and alloantigen recognition J Immunol 2007 ;  178 (7) : 4240-4249 [cross-ref]
Fenhammar J., Rundgren M., Forestier J., Kalman S., Eriksson S., Frithiof R. Toll-like receptor 4 inhibitor TAK-242 attenuates acute kidney injury in endotoxemic sheep Anesthesiology 2011 ;  114 : 1130-1137 [cross-ref]
Jiang J., Arp J., Kubelik D., Zassoko R., Liu W., Wise Y., et al. Induction of indefinite cardiac allograft survival correlates with toll-like receptor 2 and 4 downregulation after serine protease inhibitor-1 (Serp-1) treatment Transplantation 2007 ;  84 : 1158 [cross-ref]
Rice T.W., Wheeler A.P., Bernard G.R., Vincent J.L., Angus D.C., Aikawa N., et al. A randomized, double-blind, placebo-controlled trial of TAK-242 for the treatment of severe sepsis Crit. Care Med 2010 ;  38 : 1685-1694 [inter-ref]
Imamura R., Moriyama T., Isaka Y., Namba Y., Ichimaru N., Takahara S., et al. Erythropoietin protects the kidneys against ischemia reperfusion injury by activating hypoxia inducible factor- 1alpha Transplantation 2007 ;  83 : 1371 [cross-ref]
Imamura R., Isaka Y., Ichimaru N., Takahara S., Okuyama A. Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis Biochem Biophys Res Commun 2007 ;  353 : 786 [cross-ref]
Tullius S.G., Nieminen-Kelha'M, Buelow R., Reutzel-Selke A., Martins P.N., Pratschke J., et al. Inhibition of ischemia/reperfusion injury and chronic graft deterioration by a single-donor treatment with cobalt-protoporphyrin for the induction of heme oxygenase-1 Transplantation 2002 ;  74 : 591 [cross-ref]
Kotsch K., Francuski M., Pascher A., Klemz R., Seifert M., Mittler J., et al. Improved long-term graft survival after HO-1 induction in brain-dead donors Am J Transplant 2006 ;  6 : 477 [cross-ref]
Thakar C.V., Zahedi K., Revelo M.P., Wang Z., Burnham C.E., Barone S., et al. Identification of thrombospondin 1 (TSP-1) as a novel mediator of cell injury in kidney ischemia J. Clin Invest 2005 ;  115 : 3451-3459






© 2014 
Elsevier Masson SAS. Tous droits réservés.